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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
07/02/2017 |
Actualizado : |
07/04/2017 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Nacionales |
Autor : |
OLIVERA, L.; RODRÍGUEZ, E.; CERETTA, S.; BEYHAUT, E. |
Afiliación : |
LOURDES OLIVERA, Técnico Contratado por INIA; ETHEL RODRÍGUEZ, MGAP/ DGSA (DIRECCIÓN GENERAL DE SERVICIOS AGRICOLAS); SERGIO EDUARDO CERETTA SORIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ELENA BEYHAUT GUTIERREZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Repelentes de aves aplicados a la semilla de soja: compatibilidad con el inoculante y residualidad en cotiledones. (Bird Repellent Applied to Soybean Seed: Compatibility with Inoculant and Residuality in Cotyledons) |
Fecha de publicación : |
2016 |
Fuente / Imprenta : |
Agrociencia Uruguay, 2016, v.20 (2), p.51-60. |
ISSN : |
1510-0839 |
Idioma : |
Español |
Contenido : |
RESUMEN.
Tres especies de palomas causan significativas pérdidas económicas en soja (Glycine max [L.] Merr), por consumo total o parcial de cotiledones durante la emergencia del cultivo. Los repelentes son una alternativa no letal para disminuir esos daños, evitando que las aves se alimenten del cultivo. Este trabajo tuvo como objetivos conocer si repelentes aplicados a las semillas poseen la capacidad de ingresar y permanecer en los cotiledones. Los ensayos evaluaron sus efectos en la sobrevivencia de rizobios sobre las semillas y la capacidad de estos de nodular y fijar nitrógeno. Se evaluó Draza® (methiocarb 50 %), Avipel® (antraquinona 50 %) y Rayén® (metil antranilato 30 %). Las semillas fueron tratadas con los repelentes y posteriormente con inoculantes formulados en soporte líquido y turba. Se evaluó el número de rizobios viables sobre semillas a los dos y siete días luego de tratadas, y se determinaron parámetros de nodulación y peso seco de parte aérea en invernáculo. Se evaluó el contenido (ppm) de antraquinona y metil antranilato en cotiledones. La sobrevivencia de rizobios disminuyó con metiocarb y antraquinona pero no con metil antranilato. La capacidad de nodular de los rizobios y la fijación biológica de nitrógeno (FBN) fueron afectadas negativamente por el uso de metiocarb y antraquinona, siendo esta última menos perjudicial. Se observó presencia del repelente en cotiledones para antraquinona aunque no para metil antranilato. Futuras investigaciones deberían estar dirigidas a incrementar la cantidad de metil antranilato presente en cotiledones, sin afectar los rizobios.
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SUMMARY.
Three species of doves cause significant economic losses in soybean (Glycine max [L.] Merr), for total or partial consumptionof cotyledons during crop emergence. Repellents are a nonlethal alternative to reduce this damage, preventing birds from feeding on the crop. This study assessed the performance of seed-applied bird repellents. Tests involved repellents ability on reaching and staying in the cotyledons, and their effect on the survival of rhizobia on seed, nodulation and nitrogen fixation. We worked with Draza® (50 % methiocarb), Avipel® (50 % anthraquinone) and Rayén® (methyl anthranilate 30 %). Seeds were treated with the repellents and then inoculated with either liquid or peat-based formulations). The number of viable rhizobia on the seeds was measured two and seven days after treatment; and nodulation parameters and dry weight of aerial part in hothouse were determined. The content (ppm) of anthraquinone and methyl anthranilate in cotyledons was evaluated. The
survival of rhizobia decreased whith methiocarb and anthraquinone but not with methyl anthranilate. Rhizobia nodulation ability and biological nitrogen fixation (BNF) were negatively affected by the use of methiocarb and anthraquinone, the latter being less harmful. Residuality of repellent on cotyledons was observed for anthraquinone but not for methyl anthranilate. Future researchshould be aimed at increasing the amount of methyl anthranilate present in cotyledons, without affecting the rhizobia. MenosRESUMEN.
Tres especies de palomas causan significativas pérdidas económicas en soja (Glycine max [L.] Merr), por consumo total o parcial de cotiledones durante la emergencia del cultivo. Los repelentes son una alternativa no letal para disminuir esos daños, evitando que las aves se alimenten del cultivo. Este trabajo tuvo como objetivos conocer si repelentes aplicados a las semillas poseen la capacidad de ingresar y permanecer en los cotiledones. Los ensayos evaluaron sus efectos en la sobrevivencia de rizobios sobre las semillas y la capacidad de estos de nodular y fijar nitrógeno. Se evaluó Draza® (methiocarb 50 %), Avipel® (antraquinona 50 %) y Rayén® (metil antranilato 30 %). Las semillas fueron tratadas con los repelentes y posteriormente con inoculantes formulados en soporte líquido y turba. Se evaluó el número de rizobios viables sobre semillas a los dos y siete días luego de tratadas, y se determinaron parámetros de nodulación y peso seco de parte aérea en invernáculo. Se evaluó el contenido (ppm) de antraquinona y metil antranilato en cotiledones. La sobrevivencia de rizobios disminuyó con metiocarb y antraquinona pero no con metil antranilato. La capacidad de nodular de los rizobios y la fijación biológica de nitrógeno (FBN) fueron afectadas negativamente por el uso de metiocarb y antraquinona, siendo esta última menos perjudicial. Se observó presencia del repelente en cotiledones para antraquinona aunque no para metil antranilato. Futuras investigaciones deberían ... Presentar Todo |
Palabras claves : |
BIOLOGICAL N2 FIXATION; BIRD REPELLENTS; GLYCINE MAX; REPELENTE DE AVES; RHIZOBIA. |
Thesagro : |
FIJACIÓN BIOLÓGICA DEL NITRÓGENO; GLYCINE MAX; REPELENTES; RIZOBIOS; SOJA. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/6468/1/Agrociencia-2016-v.20-2-p.51-60-Ceretta-S.yBeyhaut-E..pdf
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Marc : |
LEADER 04106naa a2200289 a 4500 001 1056642 005 2017-04-07 008 2016 bl uuuu u00u1 u #d 022 $a1510-0839 100 1 $aOLIVERA, L. 245 $aRepelentes de aves aplicados a la semilla de soja$bcompatibilidad con el inoculante y residualidad en cotiledones. (Bird Repellent Applied to Soybean Seed: Compatibility with Inoculant and Residuality in Cotyledons)$h[electronic resource] 260 $c2016 520 $aRESUMEN. Tres especies de palomas causan significativas pérdidas económicas en soja (Glycine max [L.] Merr), por consumo total o parcial de cotiledones durante la emergencia del cultivo. Los repelentes son una alternativa no letal para disminuir esos daños, evitando que las aves se alimenten del cultivo. Este trabajo tuvo como objetivos conocer si repelentes aplicados a las semillas poseen la capacidad de ingresar y permanecer en los cotiledones. Los ensayos evaluaron sus efectos en la sobrevivencia de rizobios sobre las semillas y la capacidad de estos de nodular y fijar nitrógeno. Se evaluó Draza® (methiocarb 50 %), Avipel® (antraquinona 50 %) y Rayén® (metil antranilato 30 %). Las semillas fueron tratadas con los repelentes y posteriormente con inoculantes formulados en soporte líquido y turba. Se evaluó el número de rizobios viables sobre semillas a los dos y siete días luego de tratadas, y se determinaron parámetros de nodulación y peso seco de parte aérea en invernáculo. Se evaluó el contenido (ppm) de antraquinona y metil antranilato en cotiledones. La sobrevivencia de rizobios disminuyó con metiocarb y antraquinona pero no con metil antranilato. La capacidad de nodular de los rizobios y la fijación biológica de nitrógeno (FBN) fueron afectadas negativamente por el uso de metiocarb y antraquinona, siendo esta última menos perjudicial. Se observó presencia del repelente en cotiledones para antraquinona aunque no para metil antranilato. Futuras investigaciones deberían estar dirigidas a incrementar la cantidad de metil antranilato presente en cotiledones, sin afectar los rizobios. .-.-.-.-.-.-.-.-.-.-.-.-.-.-.--.-.-. SUMMARY. Three species of doves cause significant economic losses in soybean (Glycine max [L.] Merr), for total or partial consumptionof cotyledons during crop emergence. Repellents are a nonlethal alternative to reduce this damage, preventing birds from feeding on the crop. This study assessed the performance of seed-applied bird repellents. Tests involved repellents ability on reaching and staying in the cotyledons, and their effect on the survival of rhizobia on seed, nodulation and nitrogen fixation. We worked with Draza® (50 % methiocarb), Avipel® (50 % anthraquinone) and Rayén® (methyl anthranilate 30 %). Seeds were treated with the repellents and then inoculated with either liquid or peat-based formulations). The number of viable rhizobia on the seeds was measured two and seven days after treatment; and nodulation parameters and dry weight of aerial part in hothouse were determined. The content (ppm) of anthraquinone and methyl anthranilate in cotyledons was evaluated. The survival of rhizobia decreased whith methiocarb and anthraquinone but not with methyl anthranilate. Rhizobia nodulation ability and biological nitrogen fixation (BNF) were negatively affected by the use of methiocarb and anthraquinone, the latter being less harmful. Residuality of repellent on cotyledons was observed for anthraquinone but not for methyl anthranilate. Future researchshould be aimed at increasing the amount of methyl anthranilate present in cotyledons, without affecting the rhizobia. 650 $aFIJACIÓN BIOLÓGICA DEL NITRÓGENO 650 $aGLYCINE MAX 650 $aREPELENTES 650 $aRIZOBIOS 650 $aSOJA 653 $aBIOLOGICAL N2 FIXATION 653 $aBIRD REPELLENTS 653 $aGLYCINE MAX 653 $aREPELENTE DE AVES 653 $aRHIZOBIA 700 1 $aRODRÍGUEZ, E. 700 1 $aCERETTA, S. 700 1 $aBEYHAUT, E. 773 $tAgrociencia Uruguay, 2016$gv.20 (2), p.51-60.
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha actual : |
21/02/2014 |
Actualizado : |
13/09/2018 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - A |
Autor : |
NOYES, N.R.; WEINROTH, M.E.; PARKER, J.K.; DEAN, C.J.; LAKIN, S.M.; RAYMOND, R.A.; ROVIRA, P.J.; DOSTER, E.; ABDO, Z.; MARTIN, J.N.; JONES, K.L.; RUIZ, J.; BOUCHER, C.A.; BELK, K.E.; MORLEY, P.S. |
Afiliación : |
NOELLE R. NOYES; MAGGIE E. WEINROTH; JENNIFER K. PARKER; CHRIS J. DEAN; STEVEN M. LAKIN; ROBERT A. RAYMOND; PABLO JUAN ROVIRA SANZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ENRIQUE DOSTER; ZAID ABDO; JENNIFER N. MARTIN; KENNETH L. JONES; JAIME RUIZ; CHRISTINA A. BOUCHER; KEITH E. BELK; PAUL S. MORLEY. |
Título : |
Enrichment allows identification of diverse, rate elements in metagenomic resistome-virulome sequencing. |
Fecha de publicación : |
2017 |
Fuente / Imprenta : |
Microbiome, 2017, 5, p. 142 |
Páginas : |
13 p. |
DOI : |
10.1186/s40168-017-0361-8 |
Idioma : |
Inglés |
Notas : |
Article History: Received: 29 May 2017, Accepted: 5 October 2017, Published: 17 October 2017 |
Contenido : |
Background: Shotgun metagenomic sequencing is increasingly utilized as a tool to evaluate ecological-level dynamics of antimicrobial resistance and virulence, in conjunction with microbiome analysis. Interest in use of this method for environmental surveillance of antimicrobial resistance and pathogenic microorganisms is also increasing. In published metagenomic datasets, the total of all resistance- and virulence-related sequences accounts for < 1% of all sequenced DNA, leading to imitations in detection of low-abundance resistome-virulome elements. This study describes the extent and composition of the low-abundance portion of the resistome-virulome, using a bait-capture and enrichment system that incorporates unique molecular indices to count DNA molecules and correct for enrichment bias.
Results: The use of the bait-capture and enrichment system significantly increased on-target sequencing of the resistome-virulome, enabling detection of an additional 1441 gene accessions and revealing a low-abundance portion of the resistome-virulome that was more diverse and compositionally different than that detected by more traditional
metagenomic assays. The low-abundance portion of the resistome-virulome also contained resistance genes with public health importance, such as extended-spectrum betalactamases, that were not detected using traditional shotgun metagenomic sequencing. In addition, the use of the bait-capture and enrichment system enabled identification of rare resistance gene haplotypes that were used to discriminate between sample origins.
Conclusions: These results demonstrate that the rare resistome-virulome contains valuable and unique information that can be utilized for both surveillance and population genetic investigations of resistance. Access to the rare resistomevirulome using the bait-capture and enrichment system validated in this study can greatly advance our understanding of
microbiome-resistome dynamics. MenosBackground: Shotgun metagenomic sequencing is increasingly utilized as a tool to evaluate ecological-level dynamics of antimicrobial resistance and virulence, in conjunction with microbiome analysis. Interest in use of this method for environmental surveillance of antimicrobial resistance and pathogenic microorganisms is also increasing. In published metagenomic datasets, the total of all resistance- and virulence-related sequences accounts for < 1% of all sequenced DNA, leading to imitations in detection of low-abundance resistome-virulome elements. This study describes the extent and composition of the low-abundance portion of the resistome-virulome, using a bait-capture and enrichment system that incorporates unique molecular indices to count DNA molecules and correct for enrichment bias.
Results: The use of the bait-capture and enrichment system significantly increased on-target sequencing of the resistome-virulome, enabling detection of an additional 1441 gene accessions and revealing a low-abundance portion of the resistome-virulome that was more diverse and compositionally different than that detected by more traditional
metagenomic assays. The low-abundance portion of the resistome-virulome also contained resistance genes with public health importance, such as extended-spectrum betalactamases, that were not detected using traditional shotgun metagenomic sequencing. In addition, the use of the bait-capture and enrichment system enabled identification of rare resistan... Presentar Todo |
Palabras claves : |
ANTIMICROBIAL RESISTANCE; METAGENÓMICA; MICROBIAL ECOLOGY; MOLECULAR ENRICHMENT; RARE MICROBIOME; RESISTOME. |
Thesagro : |
ANALISIS BIOLOGICO; ECOLOGIA MICROBIANA; RESISTENCIA A AGENTES DANINOS. |
Asunto categoría : |
U30 Métodos de investigación |
Marc : |
LEADER 03225naa a2200433 a 4500 001 1032862 005 2018-09-13 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1186/s40168-017-0361-8$2DOI 100 1 $aNOYES, N.R. 245 $aEnrichment allows identification of diverse, rate elements in metagenomic resistome-virulome sequencing.$h[electronic resource] 260 $c2017 300 $a13 p. 500 $aArticle History: Received: 29 May 2017, Accepted: 5 October 2017, Published: 17 October 2017 520 $aBackground: Shotgun metagenomic sequencing is increasingly utilized as a tool to evaluate ecological-level dynamics of antimicrobial resistance and virulence, in conjunction with microbiome analysis. Interest in use of this method for environmental surveillance of antimicrobial resistance and pathogenic microorganisms is also increasing. In published metagenomic datasets, the total of all resistance- and virulence-related sequences accounts for < 1% of all sequenced DNA, leading to imitations in detection of low-abundance resistome-virulome elements. This study describes the extent and composition of the low-abundance portion of the resistome-virulome, using a bait-capture and enrichment system that incorporates unique molecular indices to count DNA molecules and correct for enrichment bias. Results: The use of the bait-capture and enrichment system significantly increased on-target sequencing of the resistome-virulome, enabling detection of an additional 1441 gene accessions and revealing a low-abundance portion of the resistome-virulome that was more diverse and compositionally different than that detected by more traditional metagenomic assays. The low-abundance portion of the resistome-virulome also contained resistance genes with public health importance, such as extended-spectrum betalactamases, that were not detected using traditional shotgun metagenomic sequencing. In addition, the use of the bait-capture and enrichment system enabled identification of rare resistance gene haplotypes that were used to discriminate between sample origins. Conclusions: These results demonstrate that the rare resistome-virulome contains valuable and unique information that can be utilized for both surveillance and population genetic investigations of resistance. Access to the rare resistomevirulome using the bait-capture and enrichment system validated in this study can greatly advance our understanding of microbiome-resistome dynamics. 650 $aANALISIS BIOLOGICO 650 $aECOLOGIA MICROBIANA 650 $aRESISTENCIA A AGENTES DANINOS 653 $aANTIMICROBIAL RESISTANCE 653 $aMETAGENÓMICA 653 $aMICROBIAL ECOLOGY 653 $aMOLECULAR ENRICHMENT 653 $aRARE MICROBIOME 653 $aRESISTOME 700 1 $aWEINROTH, M.E. 700 1 $aPARKER, J.K. 700 1 $aDEAN, C.J. 700 1 $aLAKIN, S.M. 700 1 $aRAYMOND, R.A. 700 1 $aROVIRA, P.J. 700 1 $aDOSTER, E. 700 1 $aABDO, Z. 700 1 $aMARTIN, J.N. 700 1 $aJONES, K.L. 700 1 $aRUIZ, J. 700 1 $aBOUCHER, C.A. 700 1 $aBELK, K.E. 700 1 $aMORLEY, P.S. 773 $tMicrobiome, 2017, 5, p. 142
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